Coding

Part:BBa_K5063013:Design

Designed by: Wong Chung Kiu   Group: iGEM24_HK-United   (2024-09-30)


GFP_TP1_insert_sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865


Design Notes

The green fluorescent protein (GFP), is a secondary fluorescent protein which produces green light while doing an energy transfer reaction. GFP is widely used as a marker of gene expression and protein localization in living and fixed tissues due to its intense fluorescence. His-tag is chosen as the affinity tag for our fusion protein due to its small size. This prevents the function or structure of our protein from being interfered. His-tags also provide inexpensive methods to perform purification. T7 promoter can be recognized by T7 RNA polymerase, a very active enzyme. The T7 RNA Polymerase/Promoter System provides a convenient method for expression in a recombinant protein. The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.



Source

[1] Leong, D. P., Joseph, P. G., McKee, M., Anand, S. S., Teo, K. K., Schwalm, J. D., & Yusuf, S. (2017). Reducing the global burden of cardiovascular disease, part 2: prevention and treatment of cardiovascular disease. Circulation research, 121(6), 695-710.https://www.ahajournals.org/doi/epub/10.1161/CIRCRESAHA.117.311849 [2] Loo, S., Kam, A., Dutta, B., Zhang, X., Feng, N., Sze, S. K., ... & Tam, J. P. (2024). Broad-spectrum ginsentides are principal bioactives in unraveling the cure-all effects of ginseng. Acta Pharmaceutica Sinica B, 14(2), 653-666.https://www.sciencedirect.com/science/article/pii/S2211383523004227 [3] Dutta B, Loo S, Kam A, Sze SK, Tam JP. Ginsentide TP1 Protects Hypoxia-Induced Dysfunction and ER Stress-Linked Apoptosis. Cells. 2023 May 16;12(10):1401. doi: 10.3390/cells12101401. PMID: 37408235; PMCID: PMC10216702.

References